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Figure 5: In situ hybridization for α1-globin, α2-globin, and β-globin in kidney of FW- and SW-adapted flounder. Abundant α1-globin and β-globin gene expression in haematopoietic stroma of FW-adapted flounder is shown with antisense α1-globin and β-globin 35S RNA probes, and α1-globin and β-globin mRNA was not detected in SW-adapted flounder. α2-globin mRNA was not detected in either of FW- and SW-adapted flounder kidney using an antisense α2-globin 35S RNA probe. The haematopoietic stroma with abundant globins expression are indicated by solid arrows. PT: proximal tubule.