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Evidence-Based Complementary and Alternative Medicine
Volume 2011, Article ID 973460, 12 pages
Original Article

A Water-Soluble Polysaccharide from the Fruit Bodies of Bulgaria inquinans (Fries) and Its Anti-Malarial Activity

1School of Life Sciences, Northeast Normal University, Changchun 130024, China
2Baicheng Medical College, Baicheng 137000, China

Received 21 January 2010; Accepted 19 May 2010

Copyright © 2011 Hongtao Bi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A water-soluble polysaccharide (BIWS-4b) was purified from the fruit bodies of Bulgaria inquinans (Fries). It is composed of mannose (27.2%), glucose (15.5%) and galactose (57.3%). Its molecular weight was estimated to be 7.4 kDa (polydispersity index, Mw/Mn: 1.35). Structural analyses indicated that BIWS-4b mainly contains ( )-linked, ( )-linked and ( ,6)-linked -Galf units; ( )-linked and non-reducing terminal -Glcp units; and ( )-linked, ( )-linked, ( ,6)-linked and non-reducing terminal -Manp units. When examined by the 4-day method and in a prophylactic assay in mice, BIWS-4b exhibited markedly suppressive activity against malaria while enhancing the activity of artesunate. Immunological tests indicated that BIWS-4b significantly enhanced macrophage phagocytosis and splenic lymphocyte proliferation in malaria-bearing mice and normal mice. The anti-malarial activity of BIWS-4b might be intermediated by enhancing immune competence and restoring artesunate-suppressed immune function. Thus, BIWS-4b is a potential adjuvant of anti-malaria drugs.