HMC05, Herbal Formula, Inhibits TNF-α-Induced Inflammatory Response in Human Umbilical Vein Endothelial Cells

Figure 1

Inhibitory effects of HMC05 and berberine on oxLDL- and TNF-α-induced monocyte adhesion to endothelial cells. The adhesion of fluorescence-labeled U937 cells to HUVECs was detected by microscopic images ((a) and (b)), and quantitated by using a fluorescence-detecting microplate reader (c). HUVECs were pretreated with different concentrations of HMC05 (1 and 10 μg ml⁻¹), simvastatin (5 μM) or berberine (1 and 10 μM) for 30 min before oxLDL (50 μg ml⁻¹) and TNF-α (10 ng ml⁻¹) treatment for 3 h. Data are expressed as the mean ± SEM of three independent experiments with duplicates. *P < .01 compared with untreated control group. ^#P < .01 compared with TNF-α-treated group.

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