Original Article

HMC05, Herbal Formula, Inhibits TNF-α-Induced Inflammatory Response in Human Umbilical Vein Endothelial Cells

Figure 3

Inhibitory effects of HMC05 and berberine on TNF-α-induced protein expression of adhesion molecules, VCAM-1 (a) and ICAM-1 (b), in HUVECs. Serum-starved HUVECs were treated with HMC05 and berberine in the absence or presence of TNF-α (10 ng ml−1) for 3 h. After fixation with methanol, immunocytochemical analysis was performed as described in ‘Materials and methods’ section. The result shown is a representative of three independent experiments.
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974728.fig.003b
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