HMC05, Herbal Formula, Inhibits TNF-α-Induced Inflammatory Response in Human Umbilical Vein Endothelial Cells
Figure 4
Inhibitory effects of HMC05 and berberine on TNF-α-induced CCR2 mRNA (a) and protein expression (b) in U937 cells. Serum-starved cells were treated with HMC05 and berberine in the presence of TNF-α for 3 h. CCR2 mRNA level was examined by real-time RT–PCR (a), and the data are expressed as the mean ± SEM of four independent experiments with duplicates. CCR2 protein expression was examined by western blot analysis (b). The blot is a representative of three independent experiments, and the bar graphs represent relative density of CCR2/Actin. Data are expressed as the mean ± SEM of three independent experiments. *P < .01 compared with untreated control group. #P < .01 compared with TNF-α-treated group.
