HMC05, Herbal Formula, Inhibits TNF-α-Induced Inflammatory Response in Human Umbilical Vein Endothelial Cells
Figure 6
Inhibitory effects of HMC05 and berberine on TNF-α-induced NF-κB activation in HUVECs. The cells were incubated with TNF-α alone for the designated time (a), or in the presence of HMC05 and berberine for 3 h (b). The nuclear extracts (20 μg protein) were separated on SDS–PAGE, transferred to a nitrocellulose membrane, and blotted with the anti-NF-κB p65 subunit antibody. The blots are a representative of three independent experiments, and the bar graphs represent relative density of NF-κB/Lamin B. (c) After HUVECs were transfected with NF-κB luciferase (firefly) construct together with pRL-TK (renilla luciferase), the cells were stimulated with TNF-α in the presence or absence of HMC05 or berberine. Luciferase activities were determined using a dual-luciferase reporter assay kit and normalized to those of control values. The data shown are mean ± SEM of three independent experiments. *P < .05 compared with untreated control group. #P < .05 compared with the TNF-α-treated group.
