KIOM-4 Protects against Oxidative Stress-Induced Mitochondrial Damage in Pancreatic β-cells via Its Antioxidant Effects
Figure 2
The effect of KIOM-4 on STZ-induced mitochondrial ROS generation. The cells were treated with KIOM-4 at 50 g ml−1. After 1 h, 10 mM of STZ was added to the plate. After an additional 30 min, the mitochondrial ROS were detected by spectrofluorometry (a) and flow cytometry (b) after DHR 123 treatment. FI indicates the fluorescence intensity of DHR 123. (c) The representative confocal images illustrate the increase in red fluorescence intensity of DHR 123 produced by ROS in STZ-treated cells s compared with that in control and the lowered fluorescence intensity in STZ-treated cells with KIOM-4 (original magnification ×400). The measurements were made in triplicate and the values were expressed as means ± SEM. Asterisk represents significantly different from control cells (P < .05) and double asterisk represent significantly different from STZ-treated cells (P < .05).
