Original Article

Anti-Cancer Effects of Protein Extracts from Calvatia lilacina, Pleurotus ostreatus and Volvariella volvacea

Figure 4

Intracellular ROS evaluation of PO and VV protein extract-treated SW480 cells. SW480 cells were exposed to PO and VV protein extracts (100  g mL−1) for 24 h. Production of intracellular ROS was detected by flow cytometry using DCFH-DA. The intracellular fluorescence of DCF was measured with a Becton-Dickinson FACScan flow cytometer. Data in each panel represent the DCF fluorescence intensity within the cells. The values shown are the means ± SD (n = 5–8 of individual experiments). (significant differences from the untreated group).
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