Inhibition of Anchorage-Independent Proliferation and G0/G1 Cell-Cycle Regulation in Human Colorectal Carcinoma Cells by 4,7-Dimethoxy-5-Methyl-l,3-Benzodioxole Isolated from the Fruiting Body of Antrodia camphorate
Figure 3
Dose-dependent SY-1-induced DNA fragmentation in human colon cancer and normal cells. (a) COLO 205 cells were treated with SY-1 and AC crude extract in a dose- and time-dependent manner. (b) COLO 205, (c) HT 29 and normal human colonic epithelial (FHC) cells were treated with SY-1 at the indicated doses. Induction of apoptosis in all cells was shown by DNA fragmentation using electrophoresis of genomic DNA. DNA fragmentation was examined 24 h after drug treatment.