Inhibition of Anchorage-Independent Proliferation and G0/G1 Cell-Cycle Regulation in Human Colorectal Carcinoma Cells by 4,7-Dimethoxy-5-Methyl-l,3-Benzodioxole Isolated from the Fruiting Body of <i>Antrodia camphorate</i>

<table>Dose effect of SY-1 on the concentrations of cell-cycle regulatory proteins. Normal human colonic epithelial (FHC, left panel) and cancer (COLO 205, right panel) cells were rendered quiescent for 24 h and then given 10% FCS in the presence or absence of SY-1 (75–150 <i>μ</i>M) for an additional 15 h. Protein extracts (100 <i>μ</i>g/lane) were separated by SDS-PAGE, probed with specific antibodies and detected using the nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate systems. Membranes were also probed with an anti-GAPDH antibody to correct for differences in protein loading.</table>

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Figure 5

Figure 5: Inhibition of Anchorage-Independent Proliferation and G0/G1 Cell-Cycle Regulation in Human Colorectal Carcinoma Cells by 4,7-Dimethoxy-5-Methyl-l,3-Benzodioxole Isolated from the Fruiting Body of <i>Antrodia camphorate</i> 

Figure 5 | Inhibition of Anchorage-Independent Proliferation and G0/G1 Cell-Cycle Regulation in Human Colorectal Carcinoma Cells by 4,7-Dimethoxy-5-Methyl-l,3-Benzodioxole Isolated from the Fruiting Body of Antrodia camphorate 