Inhibition of Anchorage-Independent Proliferation and G0/G1 Cell-Cycle Regulation in Human Colorectal Carcinoma Cells by 4,7-Dimethoxy-5-Methyl-l,3-Benzodioxole Isolated from the Fruiting Body of Antrodia camphorate
Figure 6
Anchorage-independent growth of SY-1-treated COLO 205 cells in soft-agar. (a) The COLO 205 cells were treated with SY-1 (75 μM) following methods described elsewhere [26]. The gross morphology of COLO 205 cell colonies are shown here on culture plates. These colonies exhibit subtle changes in their morphology, including a slight disaggregation compared to the untreated COLO 205 cells. Bar = 200 μM. (b) The number of colonies scored from the soft-agar plates. The COLO 205 cells were seeded in soft agar with or without SY-1 (75–375 μM). The colonies were counted in a 1 × 3 cm2 area on each plate. Data are the mean ± SE of three different experiments. Significance was accepted at P < .05. Asterisk indicates that the SY-1-treated groups were significantly different from the DMSO-treated group.
