Targeting PML-RARα and Oncogenic Signaling Pathways by Chinese Herbal Mixture Tien-Hsien Liquid in Acute Promyelocytic Leukemia NB4 Cells
Figure 4
The effects of THL on the cell-cycle regulating proteins of NB4 cells. (a) THL-induced down-regulation of cyclin A and cyclin B1 proteins of NB4 cells in a dose-dependent manner but did not significantly change the Cdc2 (CDK1) level. (b) In contrast to its effects on cyclin A and B1, THL only slightly decreased the protein levels of CDK2 and cyclin D1. The cells were treated with PBS or THL (0.375–3 mg/mL) for 72 h and then harvested. Cell lysates were then prepared for western blot analysis.
