Targeting PML-RARα and Oncogenic Signaling Pathways by Chinese Herbal Mixture Tien-Hsien Liquid in Acute Promyelocytic Leukemia NB4 Cells

Table 1

The THL-induced G2/M arrest in NB4 cells.

Treatment	Cell-cycle distribution
	Gl (%)	S (%)	G2/M (%)
PBS 24 h	48.36	32.43	19.21
PBS 48 h	48.01	28.72	23.27
THL1.5 mg/mL 24 h	11.45	38.27	50.28
THL 1.5 mg/mL 48 h	29.77	14.31	55.93
ATRA 0.25 μM 24 h	53.90	29.27	16.83
ATRA 0.25 μM 48 h	60.85	18.21	20.94

The cell-cycle distributions of THL- or ATRA-treated cells in Figure 3 were listed in the Table. THL induced G2/M arrest of cell cycle, in contrast to the G1 arrest induced by ATRA. THL, Tien-Hsien Liquid; ATRA, all-trans retinoic acid.