Validation of the Antiproliferative Effects of Organic Extracts from the Green Husk of Juglans regia L. on PC-3 Human Prostate Cancer Cells by Assessment of Apoptosis-Related Genes

Figure 2

Morphological observation with propidium iodide/FITC double staining by fluorescence microscopy (×100). Cells were cultured in six-well plates. Control cells were untreated (a, b). Test cells were subjected to treatment with IC₅₀ of methanol (c, d), chloroform (e, f), and n-hexane extracts (g, h) separately for three different incubation periods (24, 48, and 72 hours). TUNEL assay was performed and TUNEL-positive cells (green fluorescent)—the apoptotic cells—were scored out of 1000 cells counted/treated culture of PC-3 cells. Representative images from three independent experiments.

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