Involvement of Heme Oxygenase-1 Induction in the Cytoprotective and Immunomodulatory Activities of Viola patrinii in Murine Hippocampal and Microglia Cells
Figure 3
Effects of NNMBS275 on LPS-induced inducible iNOS and COX-2 protein expression and NO, PGE2, and proinflammatory cytokine levels in BV2 microglia. BV2 microglia were pretreated for 12 h with indicated concentrations of NNMBS275 and stimulated for 18 h with LPS (0.5 μg/mL). Western blot analyses for iNOS and COX-2 expression (a and b) were performed as described in Section 2. Representative blots of 3 independent experiments are shown. The concentrations of NO, PGE2, tumor necrosis TNF-α, and IL-1β (c, d, e, and f) were determined as described under Section 2. Data represent mean values of 3 experiments ± S.D. * compared to the group treated with LPS.
