Involvement of Heme Oxygenase-1 Induction in the Cytoprotective and Immunomodulatory Activities of Viola patrinii in Murine Hippocampal and Microglia Cells
Figure 7
HO-1 mediates the suppressive effect of NNMBS275 on LPS-stimulated proinflammatory mediator production, and glutamate-induced oxidative neurotoxicity. (a and b) HT22 cells were pretreated for 6 h with NNMBS275 (200 μg/mL) in the presence or absence of SnPP (50 μM), and then incubated for 12 h with glutamate (5 mM). (c, d, e, and f) BV2 cells were pretreated for 3 h with NNMBS275 (200 μg/mL) in the presence or absence of SnPP (50 μM) and stimulated for 24 h with LPS (0.5 μg/mL). The concentrations of NO, PGE2, TNF-α, and IL-1β were determined as described in Section 2. SnPP was pretreated with BV2 microglia and HT22 cells for 3 h in this experiment. Data represent mean values of 3 experiments ± S.D. *.
