Involvement of Heme Oxygenase-1 Induction in the Cytoprotective and Immunomodulatory Activities of Viola patrinii in Murine Hippocampal and Microglia Cells
Figure 9
Effects of NNMBS275 on NF-κB activation and NF-κB DNA-binding activity in BV2 cells. Cells were pretreated for 12 h with indicated concentrations of NNMBS275, and stimulated for 1 h with LPS (0.5 μg/mL). (a) Western blot analysis for IκB-α and p-IκB-α in the cytoplasm and NF-κB expression in the nucleus was performed as described in Section 2, and representative blots of 3 independent experiments are shown. (b) A commercially available NF-κB ELISA (active motif) was then used to test nuclear extracts and determine degree of NF-κB binding. Data represent the mean values of 3 experiments ± S.D. * compared to the group treated with LPS.
