Autophagy Inhibition Enhances Apoptosis Induced by Dioscin in Huh7 Cells
Figure 2
Dioscin may induce the activation of caspases in Huh7 cells. Cells were treated with an indicated concentration of dioscin for 24 hours and then analyzed by western blotting with an antibody against Bcl-2, PARP, or caspase-3 (a). Meanwhile, the expression of cleaved caspase-8, -9, and cytochrome c release was also analyzed with that of β-actin as an internal control (b). Furthermore, cells were treated with 5 μM dioscin for 24 hours in the presence or absence of 2 μM Z-DEVE-FMK, 20 μM Z-IETD-FMK, and 20 μM Z-LEHD-FMK and then subjected to MTT assay for cell viability (c). Results are shown as mean ± SD from 3 determinations per condition repeated 3 times. ***, control versus dioscin; #, dioscin versus Z-DEVE-FMK, Z-IETD-FMK, and Z-LEHD-FMK plus dioscin.
