Figure 5: Inhibition of iNOS protein expression by CRMeOH induced by 𝜆 -carrageenan (Carr) in mice paw edema for 4 h. Tissue suspended were then prepared and subjected to western blotting using an antibody specific for iNOS. 𝛽 -actin was used as an internal control. (a) Representative western blot from two separate experiments is shown. (b) Relative iNOS protein levels were calculated with reference to Carr-injected mouse. The data were presented as mean ± S.D. for three different experiments performed in triplicate. ** 𝑃 < 0 . 0 1 and *** 𝑃 < 0 . 0 0 1 were compared with Carr-alone group.