Erectogenic Effects of Clerodendron capitatum: Involvement of Phosphodiesterase Type-5 Inhibition

Table 1

The effect of C. capitatum extract on phosphordiesterase-5 in vitro towards p-nitrophenyl phenyl phosphate.

Treatment	% of relaxation*
C. capitatum 0.30 mg/mL	75.1^a±2.7
C. capitatum 0.15 mg/mL	43^b±1.9
C. capitatum 0.075 mg/mL	34^c±2.5
C. capitatum 0.04 mg/mL	31^d±4.9
C. capitatum 0.02 mg/mL	15^e±3.7
Sildenafil 0.10 mg/mL	79^a±13.2

The controls with vehicle did not show any significant effect. Using ordinary least squares regression, double reciprocal plot was performed for the enzyme inhibition (1/Extract concentration versus 1/Absorbance) to calculate the ID₅₀ and dose dependency.