Synergistic Cytotoxic Effects of Ganoderma lucidum and Bacillus Calmette Guérin on Premalignant Urothelial HUC-PC Cells and Its Regulation on Proinflammatory Cytokine Secretion
Figure 1
The treatment schedules for experiments, illustrating 4 treatments (T1–T4) were tested. A 24-hour incubation was allowed for stabilizing the culture environment after seeding the cells. The initial cell seeding numbers of T1–T3 were halves of T4, because cell cultures for T1–T3 were maintained for an additional 24 hours in complete media, in order to align initial cell numbers for all treatment groups before the first day sampling (Day 0 as baseline), that is, approximately cells since doubling time for the HUC-PC cells was 24 hours. For T4, a range of GLe concentrations was administrated before the baseline, and this complete medium in the absence of GLe (0 μg/mL) was used as the baseline for this treatment group. Subsequent samples were collected on Day 1 (Day 3 after initial cell seeding) and Day 6 (Day 8 after initial cell seeding) following different treatments.
