Synergistic Cytotoxic Effects of Ganoderma lucidum and Bacillus Calmette Guérin on Premalignant Urothelial HUC-PC Cells and Its Regulation on Proinflammatory Cytokine Secretion
Figure 2
Showing the immediate and progressive cytotoxic effects exhibited by (a) BCG on day 1 (F = 4.908; statistically nonsignificant) and day 6 (F = 51.30; ***P < 0.001), and (b) GLe on day 1 (F = 18.69; ***P < 0.001) and day 6 (F = 118.5; ***P < 0.001). Results of BCG and GLe were statistically compared with the solvent media control according to the corresponding schedule (i.e., day 1 versus Day 1 and Day 6 versus Day 6), at 0 CFU containing BCG diluents (33% v/v) and at 0 μg/mL containing ethanol (0.1%; v/v), respectively. All experiments were performed in triplicate for reproducibility. F value of each treatment group was determined by one-way ANOVA, and Dunnett’s posttest was followed to determine P values.
