Synergistic Cytotoxic Effects of Ganoderma lucidum and Bacillus Calmette Guérin on Premalignant Urothelial HUC-PC Cells and Its Regulation on Proinflammatory Cytokine Secretion
Figure 3
Showing the immediate and progressive cytotoxic effects exhibited by (a) coincubation of GLe with BCG on day 1 (F = 14.14; ***P < 0.01) and day 6 (F = 217.6; ***P < 0.001), and (b) pretreatment of GLe followed by BCG on day 1 (F = 169.8; ***P < 0.001) and day 6 (F = 30.52; ***P < 0.01). For all test conditions, BCG concentration was fixed at CFU for testing different concentrations of GLe. Results were statistically compared with control at 0 GLe concentration but treated with BCG at CFU, according to the corresponding schedule (i.e., day 1 versus day 1 and day 6 versus day 6). All experiments were performed in triplicate for reproducibility. F value of each treatment group was determined by one-way ANOVA, and Dunnett’s posttest was followed to determine P values.
