Evidence for Inhibitory Effects of Flupirtine, a Centrally Acting Analgesic, on Delayed Rectifier K+ Currents in Motor Neuron-Like Cells
Figure 3
Lack of effect of NMDA or gabazine on Flu-induced effect on in NSC-34 cells. In these experiments, each cell was depolarized from −50 to +50 mV with a duration of 10 sec. Current amplitude at the end of depolarizing pulse was measured and current inactivation time course was fitted by a single exponential. Bar graphs in (a) and (b) show the effects of Flu (3 M), Flu (3 M) plus NMDA (30 M), and Flu (3 M) plus gabazine (10 mM) on the peak amplitude (a) and inactivation time constant (b) of , respectively. In the experiments with Flu plus NMDA or gabazine, NMDA (30 M) or gabazine (10 M) was applied after the addition of Flu (3 M). Each bar represents the mean ± SEM (–12). *Significantly different from controls. Notably, subsequent application of NMDA or gabazine did not alter the amplitude and inactivation time course of .
