Table 1: Primer sequences, length of PCR products and optimal annealing temperature for each gene used in real-time quantitative PCR.

PrimerSequence (5′-3′)SourcebpTa
(°C)

TH-SCCA GTT CTC CCA GGA CAT TGG ACNM_012740.331259
TH-ASGAG GCA TAG TTC CTG AGC TTG TCC
DBH-SGAA GAA TGC TGT GAC TGT CCA CCA GNM_013158.238759
DBH-ASCAG AGG CTG CAG GTT CCA GTT AC
AADC-SGTT GTC ACC CTA GGA ACC ACA TCT TGNM_012545.344459
AADC-ASCTC ATG AGA CAG CTT CAC GTG CTT TC
MAOA-SGCC AAA GTT CTG GGA TCT CAA GAA GCNM_033653.120459
MAOA-ASCAC CAG TGA TCT TGA GCA GAC CAG
MAOB-SGAG AAG AAC TGG TGT GAG GAG CAGNM_013198.134259
MAOB-ASAGC TGT TGC TGA CAA GAT GGT GGT
COMT-SGGT GAC GCG AAA GGC CAA ATC ATGNM_012531.235159
COMT-ASCAG GCC ACA TTT CTC CAG GAG AAG
DAT-SGGT TCT ACG GCG TCC AGC AAT TCM80570.129159
DAT-ASCAT AGG CCA GTT TCT CCC GGA AG
VMAT2-SGGT GGA CTC CTC TAT GAT GCC TAT CNM_013031.335159
VMAT2-ASCTC CTT AGC AGG TGG ACT TCG AAG
NET-SCAG GTT CAG CAA TGA CAT CCA GCA GNM_031343.128259
NET-ASGTG ATT CCG TAG GCC ACT CTC TC
DrD2-SAAC TGT ACC CAC CCT GAG GAC ATGNM_012547.123659
DrD2-ASCTG TCA GGG TTG CTA TGT AGG CC
Adra1A-STGG TGG GTT GCT TCG TCC TCT GNM_017191.221159
Adra1A-ASCGA AGA CAC TGG ATT CGC AGG AC
TPH-SCAC CCA GGA TTC AAG GAC AAC GTCNM_173839.242159
TPH-ASCAC TGT GAA GCC AGA TCG CTC TTT C
SERT-SATG GTT CGT GCT CAT CGT GGT CAT CNM_013034.326859
SERT-ASGAT GAA CAG GAG AAA CAG AGG GCT G
Htr1A-SCAT CAG CAA GGA CCA CGG CTA CNM_012585.135359
Htr1A-ASGGA AGG TGC TCT TTG GAG TTG CC
NGF-SCAC TCT GAG GTG CAT AGC GTA ATG TCXP_001067130.237459
NGF-ASCTG TGA GTC CTG TTG AAG GAG ATT GTA C
BDNF-SGAG CTG AGC GTG TGT GAC AGT ATT AGBC08763422959
BDNF-ASATT GGG TAGT TCG GCA TTG CGA GTT C
GDNF-SGCG CTG ACC AGT GAC TCC AAT ATGAF49763431859
GDNF-ASCGC TTC ACA GGA ACC GCT ACA ATAT C
NT3-SACA AGC TCT CCA AGC AGA TGG TAG ATGM61179.131059
NT3-ASTCT CCT CGG TGA CTC TTA TGC TCT G
NT4-STCA GTA CTT CTT CGA GAC GCG CTGNM_013184.313559
NT4-ASGGC ACA TAG GAC TGT TTA GCC TTG CAT
NT5-SATG CAG TGA GTG GCT GGG TGA CS69323.122959
NT5-ASGTT TAG CCT TGC ATT CTG AGA GCC AG
TrkA-SACC TCA ACC GTT TCC TCC GGT CM85214.33057
TrkA-ASCTC GAT CGC CTC AGT GTT GGA GA
TrkB-SCGG GAG CAT CTC TCG GTC TAT GM55291.122157
TrkB-ASCAA ATG TGT CCG GCT TGA GCT GG
TrkC-SCAC TGT CTA CTA CCC TCC ACG TGL03813.125357
TrkC-ASCTC TCT GGA AAG GGC TCC TTA AGG
GAPDH-SAAC GGA TTT GGC CGT ATT GG Lee et al., 2009 [10]51657
GAPDH-ASCTT CCC GTT CAG CTC TGG G

Abbreviations: S: sense primer; AS: antisense primer; TH: tyrosine hydroxylase; DBH: dopamine β-hydroxylase; AADC: aromatic acid decarboxylase; MAOA: monoamine oxidase A; MAOB: monoamine oxidase B; COMT: catechol-O-methyltransferase; DAT: dopamine transporter; VMAT2: vesicular monoamine transporter 2; NET: norepinepherine transporter; DrD2: dopamine receptor D2; Adra1A: adrenergic receptor α1A; Htr 1A: serotonin receptor 1A; TPH: tryptophan hydroxylase; SERT: serotonin transporter; Htr1a: serotonin receptor 1A; NGF: nerve growth factor; BDNF: brain derived neurotrophic factor; GDNF: glial-cell-line-derived neurotrophic factor; NT3: neurotrophin 3; NT4: neurotrophin 4; NT5: neurotrophin 5; Trk A: tyrosine kinase receptor A; Trk B: tyrosine kinase receptor B; Trk C: tyrosine kinase receptor C; GAPDH: glyceraldehyde-3-phosphate dehydrogenase.