Mitigation of H2O2-Induced Mitochondrial-Mediated Apoptosis in NG108-15 Cells by Novel Mesuagenin C from Mesua kunstleri (King) Kosterm
Neuroprotective effect of mesuagenin C 3 by MTT cell viability assay. (a) Dose-dependent increase in cell viability by pretreatment with mesuagenin C 3 in H2O2-induced cell death prior to 2 mM of H2O2 exposure for 10 h. (b) Dose-dependent increase in cell viability by pretreatment with EGCG prior to 2 mM of H2O2 exposure for 10 h. (c) Comparison of mesuagenin C 3 and EGCG pretreated cell viability when exposed to H2O2. NG108-15 cells were incubated for 48 h followed by pretreatment with mesuagenin C 3 (50 μM) for 2 h prior to exposure to 2 mM H2O2 (10 h). EGCG (50 μM) was used as a standard reference. Values are mean ± S.E. from at least three independent experiments. The asterisk indicated significantly different values from H2O2-treated cells (*).