Figure 8: Flow cytometry analysis showing alterations in the of NG108-15 cells. Upon completion of treatment, the cells were stained with JC-1 and the alteration in was analyzed as mentioned in methods. (a) Representative dot plots of JC-1 aggregates (FL-2 Red fluorescence) versus JC-1 monomers (FL-1 green fluorescence). (b) Bar chart showing the percentages of relative fluorescence intensity of in control, H2O2-only and varying pretreatment concentrations of mesuagenin C 3 in NG108-15 cells. Values are mean ± S.E. from at least three independent experiments. The asterisk indicated significantly different values from H2O2-treated cells (* ).