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Evidence-Based Complementary and Alternative Medicine
Volume 2012, Article ID 157652, 10 pages
Research Article

Brazilian Green Propolis: Anti-Inflammatory Property by an Immunomodulatory Activity

1Department of Pathology, Laboratory of Immunophysiology, Biological and Health Sciences Center, Federal University of Maranhão (UFMA), 65085-580 São Luis, Brazil
2Apis Flora Industrial e Comercial Ltda, 14020-670 Ribeirão Preto, SP, Brazil
3Department of Science Physiology, Laboratory for Teaching and Research of Physiology, Federal University of Maranhão (UFMA), 65085-580 São Luis, Brazil

Received 6 August 2012; Accepted 20 November 2012

Academic Editor: Andrzej K. Kuropatnicki

Copyright © 2012 Joleen Lopes Machado et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The immunomodulatory and anti-inflammatory activities of green propolis extracts from Apis mellifera were investigated using acute and chronic inflammation models. Swiss mice were anesthetized and a cotton pellet granuloma was implanted in subcutaneous tissue. Then the mice were divided into six groups and received apyrogenic water or different propolis extracts by oral route (5 mg/kg). According to the treatment the groups were designated as E1A, E1B, E10, E11, and E12. The control group received apyrogenic water. The treatment was performed by six days when the mice were killed. The blood and the bronchoalveolar lavage (BAL) were collected to measure the leukocyte recruitment. In acute pulmonary inflammation, Balb/c mice received lipopolysaccharide (LPS) of Escherichia coli by intranasal route for three days. Concomitantly the mice received by oral route apyrogenic water (control) or E10 and E11 propolis extracts. BAL was performed to assess the inflammatory infiltrate and cytokine quantification. The results showed that the E11 extract has anti-inflammatory property in both models by the inhibition of proinflammatory cytokines and increase of anti-inflammatory cytokines suggesting an immunomodulatory activity.