Bioassay-Guided Isolation of Cytotoxic Cycloartane Triterpenoid Glycosides from the Traditionally Used Medicinal Plant Leea indica
Figure 9
Effect of MAA on cell cycle phase distribution in Ca Ski cells. Cells were treated with 60 μM MAA for 12–72 h. The untreated cells correspond to cells without MAA treatment at 12 h, and similar results were obtained at other incubation times. After treatment, cells were harvested, fixed, stained with PI, and analyzed by flow cytometry as described in methods. (a) Representative histograms showing cell cycle distribution. (b) Bar charts showing percentage of cells in sub-G1, G1, S, and G2/M phases of the cell. Results are mean values ± S.E. of three experiments. The percentage of the cell cycle phase in the treated cells was compared to the corresponding phase in the untreated control cells. Statistical significance is indicated by *.
