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Evidence-Based Complementary and Alternative Medicine
Volume 2012, Article ID 184346, 6 pages
Research Article

Evaluation of an Experimental Gel Containing Euclea natalensis: An In Vitro Study

1Department of Pediatric Dentistry, Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo, Al. Octávio Pinheiro Brisolla, 17012-901 Bauru, SP, Brazil
2Department of Chemistry, Laboratory of Natural Products, Federal University of São Carlos, Rodovia Washington Luiz, 13565-905 São Carlos, SP, Brazil

Received 3 August 2012; Accepted 27 September 2012

Academic Editor: Andrea Pieroni

Copyright © 2012 Sílvia Helena de Carvalho Sales-Peres et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Objective. To evaluate the effect of an experimental gel containing Euclea natalensis extract on dentin permeability. Methods. Thirty-six dentin discs, 1-mm-thick. The discs were prepared from the coronal dentin of extracted human third molars that were divided into 3 groups ( ). The dentin discs in each group were treated with the groups following experimental materials: (FG): 1.23% fluoride gel, pH 4.1; (EG): Euclea natalensis extract gel, pH 4.1; (CG): control gel, pH 4.1. The gels were applied to the occlusal slide of the dentin under the following conditions: after 37% phosphoric acid and before 6% citric acid. The hydraulic conductance (HC) of each condition was determined four times using a fluid flow apparatus (Flodec). The data were analyzed using Two-way ANOVA and Tukey's test ( ). Results. The greatest mean reduction in HC was produced in group EG dentin discs (61.2%; ). Even after acid challenge with 6% citric acid the great reduction occurred in group EG (66.0%; ) than other groups (CG-77.1%, FG-90.8%). Conclusion. E. natalensis gel not only reduced dentin permeability, but also resisted posttreatment citric acid challenge without changing its permeability. Further research has to confirm this promising result in the clinical situation.