Alstonia scholaris R. Br. Significantly Inhibits Retinoid-Induced Skin Irritation In Vitro and In Vivo
Table 1
Anti-inflammatory effect of Alstonia scholaris extract in all-trans retinoic acid treated HaCaT human keratinocytes.
Test substances
MCP-1
IL-8
pg/mL
% of inhibition
pg/mL
% of inhibition
ASE 500 ppm + ATRA 1 M
928.8 ± 64.0**
96.6
417.1 ± 29.0**
83.9
ASE 100 ppm + ATRA 1 M
1074.0 ± 82.2**
82.1
619.3 ± 44.4**
26.3
Madecassoside 500 ppm + ATRA 1 uM
1271.0 ± 69.0*
62.5
589.8 ± 31.0*
34.7
Madecassoside 100 ppm + ATRA 1 uM
1221.6 ± 100.8*
67.4
584.6 ± 70.6**
36.2
Hydrocortisone 1 ppm + ATRA 1 M
1027.6 ± 48.5**
86.8
407.7 ± 20.8*
86.6
ATRA 1 M
1898.2 ± 72.0
0
711.8 ± 27.0
0
Control
894.7 ± 141.0
100.0
360.4 ± 28.8
100.0
ASE: Alstonia scholaris extract; ATRA: all-trans retinoic acid; MCP-1: monocyte chemoattractant protein-1; IL-8: interleukin-8; Results are expressed as mean ± SD. * and ** compared with ATRA-treated group values.
