Tetraarsenic Hexoxide Induces Beclin-1-Induced Autophagic Cell Death as well as Caspase-Dependent Apoptosis in U937 Human Leukemic Cells
Figure 3
Regulation of Bcl-2 and IAP family proteins during As4O6-induced apoptosis. As4O6 increases the expressions of Bax in a dose- and time-dependent manner whereas the expressions of Bcl-2, Bad, Bcl-xL, and XIAP remain unchanged or slightly reduced. (a) The cells were treated with the indicated concentrations of As4O6 for 24 h. (b) The cells were treated with 3 μM of As4O6 for the indicated times. The cells treated with As4O6 were lysed and equal amounts of proteins were then separated by SDS-polyacrylamide gels and transferred to nitrocellulose membranes. The membranes were probed with the indicated antibodies and detected by an ECL detection system. To confirm equal loading, the blot was stripped of the bound antibody and reprobed with the anti β-Actin antibody. The results are from one representative experiment of at least two independent experiments that showed similar patterns.
