Research Article

Sarcandra glabra Extract Reduces the Susceptibility and Severity of Influenza in Restraint-Stressed Mice

Figure 1

Chemical fingerprint of SGE. Each peak of SGE in the HPLC fingerprint was identified by comparison of the retention times and UV spectra of chemically defined standard compounds. The standard compounds were isolated from SGE previously. Several batches of SGE were analyzed, and similar profiles were observed. HPLC condition was as follows: agilent series 1100 HPLC; column, Welch material XB-C18,  mm, particle size 5 mm; mobile phase A, H2O with 0.2% HAc; mobile phase B, MeOH with 0.2% HAc; elution program: 20% B in 5 min, linear gradient from 20% B to 62% B in 55 min, 100% B in 12 min; flow rate at 0.80 mL/min; detection wavelength at 330 nm; injection volume in 10 μL and oven temperature at 35°C.
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