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Evidence-Based Complementary and Alternative Medicine
Volume 2012 (2012), Article ID 254849, 14 pages
http://dx.doi.org/10.1155/2012/254849
Research Article

Inhibition of , , TNF- and iNOS EXpression by Shorea robusta L.: An Ethnomedicine Used for Anti-Inflammatory and Analgesic Activity

1ICMR Virus Unit, ID and BG Hospital, GB 4, First Floor, 57 Dr. Suresh C Banerjee Road, Beliaghata, Kolkata 700010, India
2Department of Applied Chemistry, Birla Institute of Technology, Mesra, Ranchi 835215, India
3Division of Microbiology, National Institute of Cholera and Enteric Diseases, Kolkata 700010, India
4Division of Pharmacology, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032, India

Received 7 January 2012; Revised 30 January 2012; Accepted 30 January 2012

Academic Editor: Vincenzo De Feo

Copyright © 2012 Chattopadhyay Debprasad et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This paper is an attempt to evaluate the anti-inflammatory and analgesic activities and the possible mechanism of action of tender leaf extracts of Shorea robusta, traditionally used in ailments related to inflammation. The acetic-acid-induced writhing and tail flick tests were carried out for analgesic activity, while the anti-inflammatory activity was evaluated in carrageenan-and dextran- induced paw edema and cotton-pellet-induced granuloma model. The acetic-acid-induced vascular permeability, erythrocyte membrane stabilization, release of proinflammatory mediators (nitric oxide and prostaglandin E2), and cytokines (tumor necrosis factor-α, and interleukins-1β and -6) from lipopolysaccharide-stimulated human monocytic cell lines were assessed to understand the mechanism of action. The results revealed that both aqueous and methanol extract (400 mg/kg) caused significant reduction of writhing and tail flick, paw edema, granuloma tissue formation ( ), vascular permeability, and membrane stabilization. Interestingly, the aqueous extract at 40 μg/mL significantly inhibited the production of NO and release of PGE2, TNF-α, IL-1β, and IL-6. Chemically the extract contains flavonoids and triterpenes and toxicity study showed that the extract is safe. Thus, our study validated the scientific rationale of ethnomedicinal use of S. robusta and unveils its mechanism of action. However, chronic toxicological studies with active constituents are needed before its use.