Oreocnide integrifolia Flavonoids Augment Reprogramming for Islet Neogenesis and β-Cell Regeneration in Pancreatectomized BALB/c Mice

Figure 2

(a) Hematoxylin and eosin-stained images of pancreas from sham operated mice at day 7, (b, c) pancreatectomized mice depicting large ducts at day 14, (d)–(f) Px + FRF-treated mice at day 7 after Px showing islet neogenesis in association with ducts. Note the islet-like aggregations in contact with ducts suggesting ductal origin. Black arrows denote the junction of duct and islets, (g, h) demonstrate sections of Px + FRF supplemented mice 14 days after Px. Note the larger-sized ducts associated islets with proliferating cells in both ducts and islets and (i) sections of pancreas of Px + FRF supplemented mice 21 days after Px showing many neogenic islets, budded off from the ducts and lying within the acini. Scale bar represents 50 μm. (j and k) Confocal immunostained images of Px and PX + FRF-treated mice at day 7 after Px showing Pdx-1/insulin coexpression, respectively, (l) percentage of islet cells coexpressing Pdx-1/insulin. (m and n) C-Peptide stained sections of Px and PX + FRF-treated mice at day 7 after Px, (o) percentage association of ducts to islets at different time points after Px and FRF supplementation. Scale bar represents 50 μm.