Oreocnide integrifolia Flavonoids Augment Reprogramming for Islet Neogenesis and β-Cell Regeneration in Pancreatectomized BALB/c Mice

Figure 5

Transcript levels of (a) Proinsulin-1, (b) Proinsulin-2, (c) Reg-3 alpha, (d) Reg-3 gamma, (e) Pdx-1 and (f) NgN-3 at different time points along with FRF treatment assessed using Taqman quantitative real time pcr. Results are represented as fold change over sham operated controls. The ΔΔ Ct method of relative quantification was used to determine the fold change in expression. All values are expressed as Mean + SEM (One way ANOVA), 𝑛=5-6, where; ^•𝑃<0.05, ^■𝑃<0.01, ^♦𝑃<0.001: Sham was compared to other experimental groups; 𝛿𝑃<0.001: Px day 7 was compared Px day 7 + FRF; black numbered 1 circle 𝑃<0.05, black numbered 3 circle 𝑃<0.001: Px day 21 was compared Px day 21 + FRF.

(a)

(b)

(c)

(d)

(e)

(f)