Beneficial Effect of Shikonin on Experimental Colitis Induced by Dextran Sulfate Sodium in Balb/C Mice
Figure 2
Effect of shikonin on colon length. The noncolitic group received fresh tap water ad libitum, and the colitic group received fresh tap water with 5% DSS added to the water during 7 days. Three other groups of mice received the same tap water with 5% DSS and were treated with two doses of 6.25, 12.5, or 25 mg kg−1 of shikonin on days 1 and 5 of the experiment. At the end of the experiment, all mice were killed by cervical dislocation and large intestines were removed. After washing with ice-cold phosphate-buffered saline, they were placed on filter papers and measured without the cecum. Statistical analysis was performed using one-way analysis of variance followed by Dunnett’s t-test. , versus colitic group. The results shown are representative of three independent experiments with 6 to 10 mice per group.
