Beneficial Effect of Shikonin on Experimental Colitis Induced by Dextran Sulfate Sodium in Balb/C Mice
Figure 5
Immunofluorescence detection of CD4+ lymphocytes and CD11b+ cells. Shikonin treatment reduces the presence of CD4+ lymphocytes and macrophages in the colon tissue of mice. Colons were obtained from the colitic group and from the shikonin-(25 mg kg−1) treated group and were processed for immunofluorescence analysis using CD4+ FITC-conjugated monoclonal antibody and CD11b+ APC-conjugated monoclonal antibody; representative results from four independent animals are shown. Arrows indicate the positive stained cells. Original magnification, 200×.
