Yang-Dan-Tang, Identified from 15 Chinese Herbal Formulae, Inhibits Human Lung Cancer Cell Proliferation via Cell Cycle Arrest
Figure 2
CHM Formula 14 decreased A549 cell survival without apparent apoptosis. A time course MTT assay was used to investigate the response of A549 cells to treatment with Formula 14. (a) Formula 14 treatment significantly inhibited the proliferation of A549 cells at all the time points studied. (b) Bright field images of A549 cells after 72 hr treatment with vehicle or Formula 14. The cell density of A549 cells after treatment with Formula 14 was much lower than the control group. (c) Representative data of annexin V and PI double staining show the apoptosis/necrosis status of A549 cells after 72 hr treatment with Formula 14. (d) Percentages of the double negative live cells (bottom left square) were quantified to compare the healthy population. Formula 14 has no effect on the live cell percentage. Student’s -test:,***.
