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Evidence-Based Complementary and Alternative Medicine
Volume 2012, Article ID 278273, 11 pages
Research Article

Isorhamnetin, A Flavonol Aglycone from Ginkgo biloba L., Induces Neuronal Differentiation of Cultured PC12 Cells: Potentiating the Effect of Nerve Growth Factor

Division of Life Science and Center for Chinese Medicine, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong

Received 6 March 2012; Accepted 11 April 2012

Academic Editor: Paul Siu-Po Ip

Copyright © 2012 Sherry L. Xu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary figure: The cytotoxicity of isorhamnetin in PC12 cells PC12 cells were seeded on to 96-well plate and incubated for 24 hours. After that, the cells were treated with isorhamnetin in different concentration for another 72 hours. The MTT solution was added to the cell cultures and incubated for 1 hour at 37 °C. Absorbance was measured at 570 nm in a microplate reader. Values are expressed as the % of total cell number against the control (0.02% DMSO), and in Mean ± SEM, n=4.

  1. Supplementary Figure