Myrrh Inhibits LPS-Induced Inflammatory Response and Protects from Cecal Ligation and Puncture-Induced Sepsis
Effects of myrrh on LPS-induced cytokine production. (a) The effects of myrrh on cell viability were measured by MTT assay. Peritoneal macrophages were incubated with or without Myrrh as indicated dose for 24 h. (b) The cells were treated with Myrrh as indicated dose, then stimulated with LPS (100 ng/mL) for 24 h. The supernants were harvested, then the levels of IL-1β, IL-6, and TNF-α were measured by ELISA as described in Subjects and Methods. (c) The cells were pretreated with myrrh as indicated dose for 1 h, then stimulated with LPS (100 ng/mL) for 24 h. Cells were taken for RT-PCR. Total RNA (1 μg) was prepared for the RT-PCR of IL-1β, IL-6, TNF-α. The IL-1β (126 bp), IL-6 (488 bp), and TNF-α (276 bp) were detected by agarose gel electrophoresis, as described in Subjects and Methods. Actin (514 bp) was used as loading control. The values are means ± SD of three independent experiments. * versus saline. + versus LPS.