Myrrh Inhibits LPS-Induced Inflammatory Response and Protects from Cecal Ligation and Puncture-Induced Sepsis
Effects of Myrrh on LPS-induced inflammatory mediators. Peritoneal macrophages were pretreated with myrrh as indicated dose for 1 h, then stimulated with LPS (100 ng/mL) for 24 h. (a) The effects of myrrh on LPS-induced COX-2 protein and mRNA expression, (b) PGE2 production, (c) iNOS protein and mRNA expression, and (d) NO production were measured. The cells were taken for western blot and RT-PCR. For western blot, total cellular proteins (20 μg) were resolved by SDS-PAGE, transferred to PVDF membrane, and detected with COX-2 (70 kDa) and iNOS (131 kDa) antibody, as described in Subjects and Methods. Actin (42 kDa) was used as loading control. A representative western blot of three experiments is shown. For RT-PCR, Total RNA (1 μg) was prepared for the RT-PCR of COX-2 and iNOS. The COX-2 (696 bp) and iNOS (734 bp) were detected by agarose gel electrophoresis, as described in Subjects and Methods. Actin (514 bp) was used as loading control. The supernants were harvested, then PGE2 and NO levels were measured as described in Subjects and Methods. The values are means ± SD of three independent experiments. * versus saline. + versus LPS.