Evidence-Based Complementary and Alternative Medicine / 2012 / Article / Fig 3

Research Article

Myrrh Inhibits LPS-Induced Inflammatory Response and Protects from Cecal Ligation and Puncture-Induced Sepsis

Figure 3

Effects of myrrh on LPS-induced NF-κB activation. (a) Peritoneal macrophages (  mm dish) were treated with myrrh (0.5 mg/mL) for 1 h, then stimulated with LPS as indicated time points. The cells were harvested for isolation of cytosol and nucleus. The nucleus fraction was performed by Nucleus extraction kit as described in Subjects and Methods. The cytosol was used for western blot. Total cellular proteins (20 μg) were resolved by SDS-PAGE, transferred to PVDF membrane and detected with phosphospefic Iκ-Bα  (36 kDa), anti-Iκ-Bα  (32 kDa) antibody, as described in Subjects and Methods. Actin (42 kDa) was used as loading control. A representative western blot of three experiments is shown. (b) The fractioned nucleus was assayed for DNA (NF-κB p65)-binding activity. The binding activity was measured by NF-κB p65 binding activity kit as described in Subjects and Methods. The values are means ± SD of three independent experiments. * versus saline.