Inulae Flos and Its Compounds Inhibit TNF-α- and IFN-γ-Induced Chemokine Production in HaCaT Human Keratinocytes

Table 2

Intraday and interday precision of the reference compounds.

Compound	Spiked concentration (μg/mL)	Intraday (𝑛=3)		Interday (𝑛=3)
		Detected concentration (μg/mL)	RSD (%)	Detected concentration (μg/mL)	RSD (%)
	3.5	3.42	0.35	3.41	0.18
Chlorogenic acid	7	7.30	0.42	7.37	0.53
	10	9.82	0.23	9.77	0.29
	1.5	1.44	0.45	1.45	0.67
Caffeic acid	3	3.04	0.70	3.05	0.72
	4.5	4.49	0.31	4.49	0.34
	35	34.87	3.24	35.55	0.56
1,5-Dicaffeoylquinic acid	70	70.91	0.65	71.05	0.31
	100	99.41	0.72	99.08	0.14
	5	5.15	1.03	5.18	1.48
Rutin	10	10.28	1.03	10.24	1.23
	15	14.77	0.36	14.78	0.40
	0.4	0.38	1.25	0.38	1.28
Kaempferol-3-O-glucoside	0.8	0.79	2.11	0.79	2.60
	1	1.02	1.42	1.00	2.02
	2	2.09	2.11	2.09	2.15
Quercetin	4	3.91	1.00	3.90	1.47
	6	6.03	0.25	6.03	0.43
	1.3	1.29	2.92	1.29	2.42
Luteolin	2.5	2.47	1.41	2.42	1.62
	3.5	3.53	0.37	3.56	1.09
	1.5	1.47	1.51	1.45	0.92
6-Methoxy-luteolin	3	2.88	1.40	2.89	0.16
	4	4.10	0.85	4.11	0.04

RSD: relative standard deviation (%) = (standard deviation/mean) × 100.