Clerodendrum inerme Leaf Extract Alleviates Animal Behaviors, Hyperlocomotion, and Prepulse Inhibition Disruptions, Mimicking Tourette Syndrome and Schizophrenia
Figure 3
CI extract, like antipsychotics, prevented methamphetamine-induced disruption of prepulse inhibition of acoustic startle response (PPI) in mice. (a): Effects of haloperidol and clozapine, typical and atypical antipsychotic agents, respectively, on the PPI disruption induced by methamphetamine. (b): Effects of CI extract on the PPI disruption induced by methamphetamine. The magnitude of PPI in the startle response to a 115 dB acoustic stimulation paired with a prepulse of 71 dB (71–115 dB) or 77 dB (77–115 dB) 120 ms ahead was measured as described in Section 2. Mice were pretreated with haloperidol (0.3 mg/kg, i.p.), clozapine (1 mg/kg, i.p.), CI extract (30 or 100 mg/kg, i.p.), or vehicle (i.p.) for 15 min, followed by methamphetamine (2 mg/kg, i.p.) for 10 min before the test. (c): The effect of CI Extract on PPI in mice without methamphetamine treatment. Normal mice were treated with CI extract (100 mg/kg, i.p.) or vehicle (i.p.) for 15 min before being subjected to the PPI test. The control group is the mice treated with normal saline only (without methamphetamine). **P < 0.01 versus the control group; #P < 0.05, ##P < 0.01, and ###P < 0.001 versus the vehicle group with the same acoustic stimulation protocol, 71–115 dB or 77–115 dB (Student’s t-test). N = 8.
