The Chinese Herbal Decoction Danggui Buxue Tang Inhibits Angiogenesis in a Rat Model of Liver Fibrosis
Effect of DBT and NAC on vWF and CD31 expression in CCl4-induced fibrotic liver tissue in rats, (a, b) Microvessel density in different groups. Immunofluorescence for vWF and CD31 was performed to analyze microvessel growth. In normal livers, the expression of vWF was limited to large vessels located in the periportal zone with no expression along the sinusoids, and no CD31 expression was observed. In CCl4-treated livers, increased vWF/CD31-positive microvessels were seen primarily in fibrotic areas. DBT- and NAC-treated livers showed reduced amounts of vWF/CD31-positive microvessels, which were also concentrated in fibrotic areas. A significant difference in vWF expression was seen between DBT- and NAC-treated groups. (c) A bar graph representation of microvessel density reported as the mean number of labeled vessel sections in 5 successive high-magnification fields (×400). The results are expressed as mean ± SD for three separate experiments. versus normal group, versus model group, versus NAC group.