Comprehensive Evaluation of Anti-hyperglycemic Activity of Fractionated Momordica charantia Seed Extract in Alloxan-Induced Diabetic Rats
Figure 5
(a) Determination of the anti-hyperglycemic effect and putative nature of fraction Mc-3.2 of M. charantia: Diabetic animals were treated with fraction Mc-3.2, or protease-treated fraction Mc-3.2 (Mc-3.2(P), 15 mg/kg b.wt. each)). Diabetic animals treated with equal volume of saline were included as negative controls whereas diabetic animals treated with protamine zinc insulin (10 IU/kg b.wt.) were taken as positive controls. Fasting serum glucose levels were estimated at 3 h postintraperitoneal administration. Values are plotted as mean ± SD from at least five rats in each group. indicates significance at compared with the respective group at 0 h. (b) SDS-PAGE analysis of active peak fraction Mc-3.2 under non-reducing condition. M indicates protein molecular weight markers and lane 1 represents fraction Mc-3.2 (15 μg).
