Antiangiogenic Effects and Therapeutic Targets of Azadirachta indica Leaf Extract in Endothelial Cells

Figure 5

Overexpression of HMOX1, ATF3, and EGR1 in HUVECs after treatment with 20.0 and 40.0 μg/mL of EENL for 24 hours. Protein levels were measured with specific antibodies by western blot analysis; GAPDH was the loading control. Vehicle-treated cells were used as control. The experiments were repeated in triplicates, and the representative blot was shown.