Figure 5: The effect of apamin on expression levels of VCAM-1, ICAM-1, TGF-β1, and fibronectin in atherosclerotic mice. (a) Western blot (left panel) and RT-PCR (right panel) suppressed expression levels of those protein by apamin. As a loading control, GAPDH was used to confirm equal sample loading. (b) Immunohistochemistry of ICAM-1 predominantly attenuated in the LPS/fat+Apa group. NC, normal control; Apa, normal control treated with apamin; LPS/fat, LPS injection and high-fat-dieted mice (atherosclerotic mice); LPS/fat+Apa, atherosclerotic mice treated with apamin. Scale bars, 50 μm.