MIC values and quantitative analysis of fungal cellular viability of pure substances maytenin and pristimerin front of the yeasts and filamentous pathogenic strains causing the most mycoses and evaluation of cytotoxic activity in NOK cells and selectivity index from the ratio of the IC50 and MFC.
Maytenin MIC/MFC
Pristimerin MIC/MFC
Amphotericin B reference values MIC
Itraconazole reference values MIC
Maytenin IC50 (mg/L)/SI
Pristimerin IC50 (mg/L)/SI
Candida albicans ATCC 90028
62.50/62.50
250.00/250.00
0.50–2.00
—
265.7/4.25♦
*
Candida krusei ATCC 6258
15.62/62.50
7.81/62.50
0.25–2.00
0.12–0.50
78.33/1.25♦
24.25/0.38♦
Candida parapsilosis ATCC 22019
15.62/31.25
125.00/125.00
0.25–1.00
0.06–0.25
78.33/2.50♦
*
Candida tropicalis ATCC 750
15.62/31.25
31.25/62.52
0.50–2.00
—
78.33/2.50♦
203.98/3.26♦
Candida glabrata ATCC 90030
31.25/31.25
125.00/125.00
0.25–1.00
—
203.98/6.52♦
*
Cryptococcus neoformans ATCC 90012
0.48/0.48
0.97/0.97
0.50
0.125
2.53/5.29♦
3.92/4.04♦
Cryptococcus neoformans R30
3.90/3.90
7.81/7.81
1.00
>32.00
22.18/5.68♦
24.25/3.10♦
Cryptococcus neoformans isolated 26
0.48/0.48
1.95/1.95
0.50
0.125
2.53/5.29♦
6.41/3.28♦
Cryptococcus neoformans isolated 27
0.48/0.48
3.90/3.90
0.50
0.125
2.53/5.29♦
20.10/5.15♦
Cryptococcus gattii 118
1.95/1.95
3.90/3.90
1.00
>32.00
9.84/5.05♦
20.10/5.15♦
Paracoccidioides brasiliensis Pb18
<0.12/-
<0.12/-
0.015–0.25
<0.0039
**
**
Histoplasma capsulatum ATCC 0G217B
0.97/0.97
0.48/0.48
0.06–0.25
0.25–2.00
3.70/3.81♦
6.43/13.40♦
Histoplasma capsulatum isolated M238P
0.48/0.48
0.48/0.48
0.06–0.25
0.25–2.00
2.53/5.29♦
6.43/13.40♦
Aspergillus fumigatus ATCC 7100
125/-
>250/-
0.12–2.0
0.12- > 16.00
*
*
Aspergillus niger ATCC 16404
0.97/0.97
250/250
0.12–0.5
0.12–1.00
♦3.70/3.81
*
Trichophyton rubrum clinical isolated
1.95/1.95
250/250
—
0.03–4.00
♦9.84/5.05
*
Trichophyton mentagrophytes ATCC 40131
3.9/3.9
62.5/250
—
0.03–0.25
♦22.18/5.68
150.24/0.6♦
Trichophyton mentagrophytes clinical isolated 102
3.9/3.9
125/250
—
0.03–0.25
♦22.18/5.68
*
MIC: minimum inhibitory concentration; MFC: minimum fungicide concentration. ♦ IC50/IS values in NOK cells.
* IC50/IS not apply because the MIC and MFC values are above 62.5 mg/L.
** IC50/IS not apply because the MIC and MFC values are below 0.12 mg/L. IC50: 50% inhibitory concentration. SI: selectivity index obtained from the relationship between the IC50 (NOK) by MFC for each fungi.
